Both normal metabolic activities and environmental factors such as solar UV light, radiation and industrial pollutants can cause damage to cellular DNA, resulting in as many as 1 million lesions per human cell per day. To contend with the constantly occurring DNA lesions, cells are equipped with multiple, highly conserved DNA repair pathways. Deficiencies in DNA repair are implicated in cancer, mental and developmental retardation, and premature aging. Our research is focused on elucidating the molecular mechanisms of DNA repair in living human and yeast cells. Our finding may offer valuable guidance for prevention and/or treatment of human diseases related to DNA damage and repair.
Tatum, D. and Li, S. (2011). Evidence that the histone methyltransferase Dot1 mediates global genomic repair by methylating histone H3 on lysine 79. J. Biol. Chem. 286, 17530-17535.
Tatum, D., Li, W., Placer, M. and Li, S. (2011). Diverse roles of RNA polymerase II-associated factor 1 complex in different subpathways of nucleotide excision repair. J. Biol. Chem. 286, 30304-30313.
Ding, B., LeJeune, D. and Li, S. (2010). The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J. Biol. Chem. 285, 5317-5326.
LeJeune, D., Chen, X., Ruggiero, C., Berryhill, S., Ding, B. and Li, S. (2009). Yeast Elc1 plays an important role in global genomic repair but not in transcription coupled repair. DNA Repair 8, 40-50.
Chen, X., Ding, B., LeJeune, D., Ruggiero, C. and Li, S. (2009). Rpb1 sumoylation in response to UV radiation or transcriptional impairment in yeast. PLoS ONE. 4, e5267.